The use of LC-MS/MS as a simultaneous analytical method for the determination of organophosphorus pesticides in human blood or urine in anti-food-terrorism measures was examined. Sample preparation required approximately 25 min, consisting of the addition of two volumes of methanol to blood or urine, vigorous shaking, cooling down, centrifugation and ultrafiltration. The simple reversed-phase LC-MS/MS condition required only 15 min per injection, being able to detect 47 pesticides in the blood and 46 pesticides in the urine. The average recoveries (n = 5) from the blood or the urine spiked at 50 ng/mL were 44.2-163.0% or 55.6-110.4%, respectively. The analytical method presented in this report is simple and could be applicable for any public health institution in anti-food-terrorism measures.
