Two experiments were conducted to assess the usefulness of repeated questionings for the psychophysiological detection of deception. Twenty-four female subjects were instructed to conceal the critical item which they had previously selected, and their skin conductance response (SCR) was simultaneously recorded. In Experiment 1, the amplitudes of SCR to both critical and noncritical items tended to decrease. However, responses to the critical item was consisitently larger than that to other noncritical ones during the total of nine presentations in this series where the critical item was pseud-randomly inserted among the four non-critical items. In Experiment 2, the difference of SCR to the critical and noncritical items was enhanced compared with Experiment 1 at the first exposure in three successive presentations, though this difference was diminished at the following 2nd and 3rd presentations. These results suggest that the successive presentations of stimulus under the detection of deception would be effective to discriminate the responses to critical item from noncritical items, and they are discussed in terms of stimulus significance.