Radioimmunoassay(PEG法)による血中T₃の測定

抄録

We have studied method of separation of bound and free type of T_8 by the use of PEG instead of DCC, and the results were as follows : 1) Reproducibility and recovery were also obtained good results. Mean coefficient of variation (CV) of accuracy of the measured T_5 was low level, 2.7% at 50-100ng/100ml, 3.0% at 100-300ng/100ml and 2.8% at over 300ng/100ml. Similarly, reproducibility of two different kit was 5.0%. 2) Recommended incubation time for the assay was two hours. 3) Dissociation of the PEG bound T_3 was found remarkably scarce in comparison with that of DCC bound T_3. 4) With high concentration of T_3 in serum, its value determined by PEG appeared fairly lower than T_3 value measured by DCC. 5) Analysis of sera from 24 normal subjects, 15 hyperthyroid and 6 hypothyroid patients yielded mean value of 149.8±24.4 (SD), 353.3±104.7 and 76.7±14.9 ng/100ml, respectively. T_8 levels in pregnant woman were a little higher than normal levels.