The aim of the present study was to obtain the optimal immunocytochemical staining condition for a CK5/14+p63+CK7/18 cocktail antibody.
Fine needle cytology smear slides from surgically resected breast nodules were used. Immunocytochemical slides were compared under four different antigen retrieval conditions.
Twenty minutes with pH9 retrieval produced good staining for the CK5/14+p63+CK7/18 cocktail antibody.
Only a few slides are available for breast cytology in routine practice. Multicolor immunocytochemistry using a cocktail antibody is becoming an important ancillary method.
