像走査法による細胞の分級技術に関する研究

抄録

Increasing the efficiency of hybrid generation of biological cell using a fusion apparatus requires a large number of viable protoplasts, which are classified beforehand from suspension-included viable and disintegrated biological cells. A cytometric classifier has been developed for protoplasts whose diameters are more than about ten micrometers. This paper introduces a new classification method. Protoplasts are supplied through a sheath flow in a flow cell and their images are focused on four slits arranged at different angles to streamline and scan the moving images relatively. Through these slits, three direction length of a protoplast are measured and the protoplast is classified. The classified protoplasts are sorted into microchambers in isotonic solution. Cytometric resolution of the present classifier is ±10 μm. From the experimental result, knowledge is obtained on the improvement of cytometric resolution and classifying efficiency.