抄録
Previous investigations have been demonstrated that several oxidation products derived from methyl linoleate possess the definite toxicity for mice and chick embryos in vivo.
In this study, the effect of their compounds on the (Na++K+) -ATPase (EC 3.6. 1.3) obtained from rat jejunum mucosal cell in vitro was clarified. To investigate the oil-enzyme interaction, authors proposed a new technique using the oil adsorbed on cellulose powder without surface active agents which inactivate the enzyme activity.
By this procedure, it was found that all oxidized compounds inhibited the (Na++K+) -ATPase activity more or less and their concentrations of 50% inhibition of the enzyme (Ki) corresponded closely to the LD50s for chick embryos or the decreases in the body weight of mice administered them.
Thus, the biological lesions previously found such as increase of water content in the small intestinal lumen of mouse administered could be explained by the inhibition of (Na++K+) -ATPase.
