The fatty acids in the seed oil of Lindera citriodora (Sieb, et Zucc.) Hemsi. were investigated by means of gas chromatography, column chromatography, mass, infrared and nuclear magnetic resonance spectrum. The acids identified were octanoic, decanoic, cis-4-decenoic, undecanoic, dodecanoic, cis-4-dodecenoic, tetradecenoic, cis-4-tetradecenoic, hexadecanoic, hexadecenoic, octadecanoic, oleic, linoleic and eicosenoic acid. The content of cis-4-decenoic, cis-4-dodecenoic and cis-4-tetradecenoic acid obtained from the seed collected in July, decreased compared with that collected in September, but on the contrary the content of decanoic and dodecanoic acid increased.