Analysis of Triglycerides of Soybean Oil by High-Performance Liquid Chromatography in Combination with Gas Liquid Chromatography

抄録

A technique of high-performance liquid chromatography (HPLC) was applied to the analysis of triglycerides of soybean oil. The triglycerides were clearly separated into six peaks by HPLC on a column packed with, μ-Bondapack C₁₈ using methanol-chloroform mixture as a solvent. From the fatty acid compositions of the triglycerides of these six peaks, it was found that the separation of triglycerides by HPLC under the conditions used was governed by the degree of their unsaturation in addition to carbon number ; that is, the higher the degree of unsaturation of the triglyceride, the earlier the elution. From the results obtained, the possible triglyceride compositions of soybean oil were estimated.
As a result, it was suggested that the use of HPLC in combination with GLC and TLC can provide more detailed information concerning triglyceride structure.