トリグリセドのGC-MS分析 (第1報)

総脂肪酸組成

抄録

On the analysis of triglyceride by GC-MS, it was commonly assumed that the intensity of some fragment ions (RCO⁺ and [RCO+128] ⁺ ion) were proportional to the quality of corresponding fatty acid. The propriety of the assumption was investigated.
Prepared model triglyceride mixture consisted of saturated straight chain fatty acid and had wide molecular distribution. As for total fatty acid composition, GC-MS method was compared with usual hydrolysis-GC method. GC-MS (depend on intensity of RCO⁺ ion) provided slight excess value at C₈ to C₁₄-acid, and less value at C₁₆ to C₂₀-acid. [RCO+128] ⁺ ion had same tendency as RCO⁺ ion, but it was susceptible to obstruction of [RCO+128+14n] ⁺ ion series.