Lipid-biomodification ability was examined for yeasts isolated from soil using culture medium containing beef tallow (2 %). Some yeasts, e.g. Candida, Trichosporon and Rhodotorula species were able to grow on fats and oils. Fatty acid and triacylglycerol compositions were modified in lipids of some strains. Candida sp. MIS-1 and YM1-1 preferentially produced oleic acid. Candida sp. MIS-1 had high level of triacylglycerol with a melting point like olive oil. Fatty acid composition of lipids in Candida lipolytica IAM4948 and Rhodotorula sp. A03-5 was similar to that of cacao butter. Yeast oils obtained from C. lipolytica provided the melting characterization different from beef tallow.