抄録
In order to improve transfection efficiency of the stage X blastoderm of chickens by in vivo electroporation, the effect of the size of the upper and lower electrodes was examined. The plasmid DNA (pbAEGFP) was injected into the stage X blastoderm followed by applying electric pulses vertically to the blastoderm layer. The manipulated embryos were incubated using an embryo culture system, and the GFP gene expression was then examined. By decreasing the size of the upper electrode or by increasing the size of the lower electrode, the effect of the electric pulses was clearly enhanced. Although the introduced GFP gene was efficiently expressed in the embryonic and extra-embryonic tissues, the expression in the embryos was transient. No integration of the GFP gene into the chromosomes of germline cells of chickens was detected.
