Effects of Calcium Regulating Hormones on Osteoclast-Like Cell Formation in Hen Medullary Bone Marrow Culture

抄録

In order to examine the effects of the calcium regulating hormones on the differentiation of hen medullary bone osteoclasts, isolated medullary bone marrow cells were cultured for 8 days in media including parathyroid hormone (PTH), 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃] or 17β-estradiol (E₂), respectively.
After 8 days of culture, tartrate-resistant acid phosphatase (TRAP) activity, a marker for osteoclasts, was enzymehistochemically detected on the cultured marrow cells and these cells were also stained with Mayer’s hematoxylin. Thereafter, TRAP-positive multinuclear cells were counted as osteoclast-like cells, and the effects of the calcium regulating hormones on the osteoclast differentiation from medullary bone marrow cells were indicated as the percentage (%) of the controls when vehicle was added in place of the hormones. As a result, treatment with PTH or 1,25(OH)₂D₃ significantly stimulated the differentiation of osteoclast-like cells, up to 218.3% or 159.2%, respectively. On the other hand, E2 treatment resulted in a moderate, but not significant, suppression of differentiation (85.8% of controls).
These results indicate that PTH and 1,25(OH)₂D₃ stimulate medullary bone osteoclast differentiation, but E2 is likely inhibitory.