Isolation and Partial Characterization of a Bovine Amniotic Fluid Protein

Hiroharu MATSUSHITA; Kenji TUCHIHASHI; Shuichi ASANO; Toshiho NISHITA; Manabu YAMADA; Hirohumi ANDO

doi:10.1262/jrd.39.275

Hiroharu MATSUSHITA, Kenji TUCHIHASHI, Shuichi ASANO, Toshiho NISHITA, Manabu YAMADA, Hirohumi ANDO

著者情報

キーワード: Bovine, Amniotic fluid, Pregnancy-associated protein

ジャーナルフリー

1993 年 39 巻 4 号 p. 275-279

DOI https://doi.org/10.1262/jrd.39.275

詳細

抄録

An bovine, amniotic-fluid protein, (bAP), was purified from amniotic fluid by gel filtration, anion exchange chromatography and isoelectric focusing. The isolation was monitored by a double immunodiffusion with an antiserum raised against amniotic fluid antigens and absorbed with maternal and fetal sera. The molecular weight of an amniotic fluid protein isolated was estimated as 60, 000 by SDS-PAGE, and the isoelectric point by isoelectric focusing was 3.4-3.8.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）