Nervous Necrosis Virus (NNV) -Susceptibility and -Productivity of Newly Established Cell Lines Derived from Sevenband Grouper Hyporthodus septemfasciatus

Hyun Jung Gye; Han Sol Lee; Toyohiko Nishizawa

doi:10.3147/jsfp.56.22

短報

Nervous Necrosis Virus (NNV) -Susceptibility and -Productivity of Newly Established Cell Lines Derived from Sevenband Grouper Hyporthodus septemfasciatus

Hyun Jung Gye, Han Sol Lee, Toyohiko Nishizawa

著者情報

キーワード: primary cell lines, sevenband grouper, nervous necrosis virus, NNV-productivity, growth temperature

ジャーナルフリー

2021 年 56 巻 1 号 p. 22-25

DOI https://doi.org/10.3147/jsfp.56.22

詳細

抄録

Multiple cell lines permissive to nervous necrosis virus (NNV) infection may be necessary for identifying receptor binding sites of viral surface protrusions. We have established new cell lines, SeGE-12, SeGE-22, SeGF and SeGB, derived from eyed-egg embryos, fin and brain of sevenband grouper. The optimum growth temperatures of these cell lines were all around 30°C. The growth temperature range for SeGE-12 and SeGE-22 was 25°C–32°C, whereas for SeGF and SeGB it was 15°C–32°C. These cell lines exhibited no cytopathic effect (CPE) due to NNV infection, but released extracellular NNV at 10^2.8 to 10^4.7 TCID₅₀/mL after 14 days incubation.

責任著者(Corresponding author)

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