魚病研究
Online ISSN : 1881-7335
Print ISSN : 0388-788X
ISSN-L : 0388-788X
短報
Nervous Necrosis Virus (NNV) -Susceptibility and -Productivity of Newly Established Cell Lines Derived from Sevenband Grouper Hyporthodus septemfasciatus
Hyun Jung GyeHan Sol LeeToyohiko Nishizawa
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ジャーナル 認証あり

2021 年 56 巻 1 号 p. 22-25

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Multiple cell lines permissive to nervous necrosis virus (NNV) infection may be necessary for identifying receptor binding sites of viral surface protrusions. ​We have established new cell lines, SeGE-12, SeGE-22, SeGF and SeGB, derived from eyed-egg embryos, fin and brain of sevenband grouper. ​The optimum growth temperatures of these cell lines were all around 30°C. ​The growth temperature range for SeGE-12 and SeGE-22 was 25°C–32°C, whereas for SeGF and SeGB it was 15°C–32°C. ​These cell lines exhibited no cytopathic effect (CPE) due to NNV infection, but released extracellular NNV at 102.8 to 104.7 TCID50/mL after 14 days incubation.

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© 2021 The Japanese Society of Fish Pathology
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