抄録
It has been suggested that, when in solution with a surfactant (Triton X100), Photofrin II acts like a monomer. In 1985, Dr Sakai had performed basic studies to detect singlet oxygen from HpD using the Luminol reaction method of Raubut. We observed the production of singlet oxygen from Photofrin II to which Triton was added, using the Luminol reaction. The concentration of Photofrin II was constant. Various concentrations of Triton were excited by excitation with 395nm wavelength light, and the fluorescent spectra of Photofrin II was observed. Mixtures of each solution and Luminol were excited by excitation with 395nm wavelength light. The fluorescent spectra of Luminol, aminophthalic acid and Photofrin II were measured every five minutes to evaluate the production of aminophthalic acid. Consequently the fluorescent peak of Photofrin II shifted and the fluorescent intensity of Photofrin II was double when the concentration of Triton was above 1.25×10-2%. When Luminol was added, the rate of increase of the fluorescent intensity of aminophthalic acid increased, together with the change of fluorescent spectra. Aminophthalic acid production was regarded as depending on that of singlet oxygen because the production of aminophthalic acid was suppressed in solution to which beta carotene was added.
