Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
High Genetic Diversity of Anaplasma marginale Detected from Philippine Cattle
Adrian Patalinghug YBAÑEZRochelle Haidee D. YBAÑEZFlorencia G. CLAVERIAMary Jane CRUZ-FLORESXuen XUENANNaoaki YOKOYAMAHisashi INOKUMA
ジャーナル フリー

2014 年 76 巻 7 号 p. 1009-1014


A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle.


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