2001 年 51 巻 4 号 p. 255-259
On the basis of the results of our investigation about the dialysed extract of Dermatophagoides farinae (Df) (Int Arch Allergy Clin Immunol 96 : 622-330, 1990), we studied the existence of low molecular weight fraction of Df (Df-LMN) in sera of the mite sensitive patients with bronchial asthma and with atopic dermatitis. ELISA inhibition assay and Western immunoblotting were used to compare the amount of Df-LMW in sera of 19 asthmatic patients with RAST positive for, Df, 9 asthmatic patients with RAST negative for Df, 11 patients with atopic dermatitis with RAST positive for Df, 10 healthy children with RAST negative for Df without any family history of allergic diseases. The levels of Df-LMW in sera of the asthmatic patients and atopic dermatitis patients with RAST positive for Df were significantly higher than that of healthy children with RAST negative for Df (p < 0.01). Furthermore, using Western immunoblotting, most sera from asthmatic patients with mite allergy showed positive bands, but didn't show any bands in patient without mite allergy and healthy children. Judging from maker protein, Df-LMW present around 66 and 34 Kd with coujugation of serum protein. The results of this study provide that Df-LMW may present in sera of patients with mite allergy.
