Characterization of a New DNA Polymerase Activity from Brassica oleracea, var. botrytis
2003 年 53 巻 2 号 p. 121-127
詳細
2003 年 53 巻 2 号 p. 121-127
Background & Aims : A new DNA polymerase activity (temporally designated-C) isolated from a rapidly growing apical tissue of cauliflower (Brassica oleracea var. botrytis) inflorescence has been characterized and compared with cauliflower DNA polymerases, -A and-B, and with DNA polymerases α, β and γ from mouse myeloma.
Methods : Enzyme activity was determined in vitro by measuring the incorporation of [3H] dTMP into the DNA polymer under a variety of conditions (inhibitor : NEM, ddTTP, template-primer utilities and so on).
Results : DNA polymerase-C from cauliflower had distinct elution pattern discriminated from these of polymerase-A and-B. Poly (rA) (dT) 10 was utilized as template effectively for polymerase-C, but no significant utilization of polymerase-A was observed. Polymerase-C was sensitive to NEM and was resistant to ddTTP.
Conclusions : These results strongly indicate that DNA polymerase-C from cauliflower is to be a new enzyme for DNA synthesis in cauliflower.
