関東東山病害虫研究会報
Online ISSN : 1884-2879
Print ISSN : 1347-1899
ISSN-L : 1347-1899
病害の部
enzyme-linked immunosorbent assay (ELISA) 法による植物ウイルスの検出における消泡剤の使用
奥田 充
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ジャーナル フリー

2016 年 2016 巻 63 号 p. 52-55

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The use of antifoam reagents in enzyme-linked immunosorbent assay (ELISA) was examined to reduce troubles during the assay for plant virus detection. Antifoams, SI and PE-L were added to a washing buffer (WB), phosphate buffered saline with Tween® 20 (PBST) or a chromogenic substrate, p-nitrophenylphosphate (CS) solution for alkaline phosphatase. The absorbance values for Tomato spotted wilt virus, Zucchini yellow mosaic virus and Rice stripe virus (RSV) measured no difference between PBST with/without antifoams (0.1%) used as WB although those using CS containing antifoams showed higher values. PBST with SI was also effective for the simplified procedure for extraction of RSV. In conclusion, PBST with an antifoam SI is effective to improve operation efficiencies of ELISA.

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© 2016 関東東山病害虫研究会
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