Sixty-six butterfly species have been recorded from Tatsutayama, Kumamoto City. They consisted of nine papilionid, seven pierid, 17 lycaenid, 23 nymphalid and ten hesperiid species. Six of these appeared in the record from the 1970s, but have not been observed in the 2000s. On the other hand, three Red Data species of Japan and/or Kumamoto Prefecture were observed even in the 2000s. Fifty-four species were recorded in the transect counts over three years. The most abundant species were Zizeeria maha and Eurema mandarina, followed by Ypthima argus, Argyreus hyperbius and Lethe diana. The Shannon-Wiener function (H’) was 4.00-4.34, the Sympson biodiversity index (1-λ) was 0.903-0.927, the Pielou measurement of evenness (J’) was 0.725-0.782, and the density (counts / km / census) was 10.77-15.02.