抄録
Phylogenetic relationships of important trichothecene-producing strains originally identified as Fusarium nivale Fn-2, Fn-3 and Fn-2B and F. episphaeria Fn-M were investigated within the Gibberella lineage of Fusarium using nucleotide characters obtained by sequencing polymerase chain reaction (PCR) DNA amplified from the following 4 loci: nuclear 28S ribosomal DNA, nuclear ribosomal internal transcribed spacer (ITS) region, mitochondrial small subunit (mtSSU) ribosomal DNA, and β-tubulin gene exons and introns. Parsimony analysis of the individual and combined data sets indicate that Fn-2, Fn-3, Fn-2B, and Fn-M represent an undescribed species of Fusarium. Bootstrap and decay analysis identified a clade containing F. sambucinum and several other diacetoxyscipenol (DAS) and/or T-2 trichothecene-producing species as a sister to Fusarium sp. n. The outgroup species used to root the tree, by contrast, produce a different set of mycotoxins that include deoxynivalenol and zearalenone. These results emphasize the importance of investigating the evolution of Fusarium toxins the contex of a robust species-level phylogeny.
