Bacillus subtilisPCI 219の胞子の発芽機構に関する研究(第3報)

アルカリ金属イオンならびにメチレン青の結合量と発芽との関係

抄録

The mechanism of stimulation of alkaline metals on the germination of the spore of B. subtilis PCI 219 induced by L-alanine was studied.
It was suggested that there were more than one binding site available to methylene blue (MB) on the spore and one of them (site I) was competed for binding with K⁺ or Na⁺. Association constants for K⁺, Na⁺ and MB to the site I were calculated as follows: k_K=160, k_Na=300 and k_MB=5.4×10⁵. Total number of the site I on a spore were found to be 2.6×10⁷.
In the absence of MB, the germination velocity was closely related to the amount of metal ions bound to the site I, but in the presence of MB the germination rate was affected by the amount of both cations and MB bound to the site I; the former stimulated the germination and the latter inhibited.
Results obtained showed that what was effective on the germination, was not intrinsic cations of the spore but bound cations from the external solution and suggested that the site I was negative group of the spore.