Abstract
This study described the screening of α-amylase and α-glucosidase inhibitory activity in hot water and ethanol extracts of 195 species of wild/cultivated mushrooms. Tricholoma giganteum (Nioushimeji), Russula nigricans (Kurohatsu), Morchella esculenta (Amigasatake) and so on showed potent α-amylase inhibitory activity, and Boletus pseudocalopus (Niseashibeniiguchi), Cortinarius alboviolaceus (Usufujifuusentake), Cortinarius armillatus (Tsubafuusentake), Dictyophora indusiata (Kinugasatake) and so on showed potent α-glucosidase inhibitory activity. When they are compared using IC50 data, α-glucosidase inhibition activity of these mushrooms was stronger than α-amylase inhibition activity of them. Active components of α-glucosidase inhibition activity were indentified as nojirimycin derivatives ; α-homonojirimycin and 7-o-β-D-glucopyranosyl-α-homonojirimycin, in four mushrooms such as B. pseudocalopus, C. armillatus, C. alboviolaceus, and D. indusiata using LC-TOFMS. Total nojirimycin derivative contents seemed to be correlated with α-glucosidase inhibitory activity. Therefore, it was suggested that the main active components of α-glucosidase inhibitory activity were nojirimycin derivatives regarding these four mushrooms.
