Abstract
Glycoalkaloids (GAs), e.g. α-chaconine and α-solanine, are found in potatoes. Sometimes, they cause food poisonings. We have developed easy and rapid method to detect GAs in potato using a high performance liquid chromatograph (HPLC). GAs were analysed under the following conditions; column: Inertsil C8 (250mm×4.6mmI.D.); mobile phase: 20mM phosphate buffer+acetonitrile (67:33, 0.7ml/min); wavelength to detect: 205nm. GAs were extracted with methyl alcohol and analyzed with HPLC. This method was applied to edible and other parts of potato plant.α-Chaconine was found in roots (2520mg/kg), leaves (489mg/kg), periderm tubers (263mg/kg) and stems (62mg/kg).α-Solanine was found in roots (1400mg/kg), leaves (152mg/kg), periderm tubers (109mg/kg)and stems (26mg/kg). In cortex tubers, α-chaconine and α-solanine were not found at the detection limit of 10mg/kg.
