1964 年 5 巻 5 号 p. 379-382
The coloring matters formed by the color reaction between chlorophenols and 4-aminoantipyrine were separated on silica gel.
Glass plates coated with “Silica gel G” to a thickness of 250μ were dried in an oven at 110° for 1 hour.
The mixture of the coloring matters (CHCl3 extracts, 0.01-0.03μg as each chlorophenol) was introduced by means of a micropipette at a corner 1.5cm from the lower edge of the plate and developed with the solvent mixtures (see Table 1, 2) until the solvent front reached a distance of 10cm from the starting point on the plate.
As each spot gives a red color or orange color, this method has no need of detection reagent.
The coloring matters were isolated and it was confirmed that the couplings of 4-aminoantipyrine with the chlorophenols take place in the position para to phenolic OH group by Ultraviolet absorption spectra, Visible absorption spectra, Infrared absorption spectra and thin-layer chromatography of the coloring matters.