抄録
The purpose of our research is to design a composite biofilm with nitrifying bacteria and denitrifying bacteria that imitate a biofilm in the nature. As a first step, a biofilm formation of denitrifying bacteria on a support material was examined from the viewpoint of fine particle technology. As a result, it was found that the area fraction of denitrifying bacteria adhered on the support material was related to the change in energy of interaction between denitrifying bacteria and support material, ΔG, which was calculated from their surface tensions. Furthermore, the pattern of biofilm formation was found to be depended on ΔG. These results indicate that ΔG can be treated as an indicator of biofilm formation.