血合筋肉の化学的研究-IV 血合筋肉ミオグロビンの結晶に就いて

松浦 文雄; 橋本 周久

doi:10.2331/suisan.20.946

血合筋肉の化学的研究-IV

血合筋肉ミオグロビンの結晶に就いて

松浦文雄, 橋本周久

著者情報

ジャーナルフリー

1955 年 20 巻 10 号 p. 946-950

DOI https://doi.org/10.2331/suisan.20.946

詳細

発行日: 1955 年受付日: - J-STAGE公開日: 2008/02/29 受理日: 1954/12/27 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/02/29 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : MMb crystals were obtained from the deep-seated red muscle (“Chiai”) of fishes, e. g., Parathunnus sibi, Thunnus orientalis and others, by the method of S_CHMID⁵⁾) (Fig. 1). The iron content was determined to be 0.334-0.335%, according to the D_RABKIN's o-phenanthroline method⁷⁾). In the present paper, the several derivatives of these Mb's were compared spectrophoto-metrically with those of the crystalline MMb prepared from horse heart in the same procedure. The millimolar extinction coefficients of these Mb's are tabulated in Table 2, together with the B_OWEN's data on horse heart Mb⁹⁾) and _DE D_UVE's data on human heart Mb¹⁰⁾) for reference.
Absorption maxima and minima of horse heart Mb derivatives appear very close with those of B_OWEN, although the extinction coefficients of the former are generally lower than those of the latter ; this is mainly due to the difference of basis in calculating the Mb concentration, the coefficients in this investigation being calculated on the equivalent iron basis and those of B_OWEN calculated in the other way (see the foot-note of Table 2).
The absorption spectra of these fish MbO₂ were located nearer the short wave-lengths than those of horse MbO₂, and found to be present at the same wave-lengths as HbO₂, prepared from the blood of immature Thunnus orientalis, and also at the almost same positions as HbO₂ of higher vertebrates¹³⁾) (Fig. 2). This fact goes against the usual conception that the absorption spectrum of MbO₂ is shifted toward longer wave-length than that of Hb0₂. Further, it is remarkable that the β-maximum of MbO₂ of fish is unusually higher than its α-maximum. Such a kind of absorption spectrum seems not yet to have been reported in vertebrate Mb and Hb.
In the case of MbCO of fishes too, the similar specificity was observed, i. d., positions of the maxima and minima were far from those of horse MbCO, but at the same wave-lengths as those of HbCO from fish and higher vertebrates¹³⁾) (Fig. 3).
The other derivatives, such as reduced-, met-, cyanmet-Mb, and reduced pyridine-hemochromogen of fishes and horse are nearly identical each other in the absorption spectrum (Fig. 4).
Right : MMb crystals were obtained from the deep-seated red muscle (“Chiai”) of fishes, e. g., Parathunnus sibi, Thunnus orientalis and others, by the method of S_CHMID⁵⁾ (Fig. 1). The iron content was determined to be 0.334-0.335%, according to the D_RABKIN's o-phenanthroline method⁷⁾. In the present paper, the several derivatives of these Mb's were compared spectrophoto-metrically with those of the crystalline MMb prepared from horse heart in the same procedure. The millimolar extinction coefficients of these Mb's are tabulated in Table 2, together with the B_OWEN's data on horse heart Mb⁹⁾ and _DE D_UVE's data on human heart Mb¹⁰⁾ for reference.
Absorption maxima and minima of horse heart Mb derivatives appear very close with those of B_OWEN, although the extinction coefficients of the former are generally lower than those of the latter ; this is mainly due to the difference of basis in calculating the Mb concentration, the coefficients in this investigation being calculated on the equivalent iron basis and those of B_OWEN calculated in the other way (see the foot-note of Table 2).
The absorption spectra of these fish MbO₂ were located nearer the short wave-lengths than those of horse MbO₂, and found to be present at the same wave-lengths as HbO₂, prepared from the blood of immature Thunnus orientalis, and also at the almost same positions as HbO₂ of higher vertebrates¹³⁾ (Fig. 2). This fact goes against the usual conception that the absorption spectrum of MbO₂ is shifted toward longer wave-length than that of Hb0₂. Further, it is remarkable that the β-maximum of MbO₂ of fish is unusually higher than its α-maximum. Such a kind of absorption spectrum seems not yet to have been reported in vertebrate Mb and Hb.
In the case of MbCO of fishes too, the similar specificity was observed, i. d., positions of the maxima and minima were far from those of horse MbCO, but at the same wave-lengths as those of HbCO from fish and higher vertebrates¹³⁾ (Fig. 3).
The other derivatives, such as reduced-, met-, cyanmet-Mb, and reduced pyridine-hemochromogen of fishes and horse are nearly identical each other in the absorption spectrum (Fig. 4).

訂正日: 2008/02/29 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

MMb crystals were obtained from the deep-seated red muscle (“Chiai”) of fishes, e. g., Parathunnus sibi, Thunnus orientalis and others, by the method of S_CHMID⁵⁾ (Fig. 1). The iron content was determined to be 0.334-0.335%, according to the D_RABKIN's o-phenanthroline method⁷⁾. In the present paper, the several derivatives of these Mb's were compared spectrophoto-metrically with those of the crystalline MMb prepared from horse heart in the same procedure. The millimolar extinction coefficients of these Mb's are tabulated in Table 2, together with the B_OWEN's data on horse heart Mb⁹⁾ and _DE D_UVE's data on human heart Mb¹⁰⁾ for reference.
Absorption maxima and minima of horse heart Mb derivatives appear very close with those of B_OWEN, although the extinction coefficients of the former are generally lower than those of the latter ; this is mainly due to the difference of basis in calculating the Mb concentration, the coefficients in this investigation being calculated on the equivalent iron basis and those of B_OWEN calculated in the other way (see the foot-note of Table 2).
The absorption spectra of these fish MbO₂ were located nearer the short wave-lengths than those of horse MbO₂, and found to be present at the same wave-lengths as HbO₂, prepared from the blood of immature Thunnus orientalis, and also at the almost same positions as HbO₂ of higher vertebrates¹³⁾ (Fig. 2). This fact goes against the usual conception that the absorption spectrum of MbO₂ is shifted toward longer wave-length than that of Hb0₂. Further, it is remarkable that the β-maximum of MbO₂ of fish is unusually higher than its α-maximum. Such a kind of absorption spectrum seems not yet to have been reported in vertebrate Mb and Hb.
In the case of MbCO of fishes too, the similar specificity was observed, i. d., positions of the maxima and minima were far from those of horse MbCO, but at the same wave-lengths as those of HbCO from fish and higher vertebrates¹³⁾ (Fig. 3).
The other derivatives, such as reduced-, met-, cyanmet-Mb, and reduced pyridine-hemochromogen of fishes and horse are nearly identical each other in the absorption spectrum (Fig. 4).

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