1955 年 20 巻 10 号 p. 951-954
Cytochrome c (Cyt) preparations contain _??_ about 0.34% iron were prepared from the deep-seated red muscle of tunny (Thunnus orientalis) according to the method of KEILIN and HARTREE 6), the iron being determined by DRABKIN's o-phenanthroline method11). “0.43% Fe” preparations were prepared by means of Amberlite IRC-50 after MARGOLIASH10), from the above-mentioned preparations (Table 1). At the same time, Cyt preparations were obtained from the heart muscle of horse for reference.
The spectral characters of tunny and horse Cyt's were then investigated (Fig. 1 & Table2, 3). Both in the “0.34% Fe” and in the “0.43% Fe” preparation, there were no significant differences between the spectra of these species Cyt's; i.e., the absorption bands of these Cyt's appear at the same wave-lengths and their spectrophotometric constants are fairly close each other.