イカ肉水抽出液中のM-アクトミオシンと塩類抽出液中のアクトミオシン様蛋白との異同

抄録

1) Some electrophoretic study was made on M-actomyosin, an actomyosin-like protein obtained from aqueous extract of the squid muscle. The actomyosin-like pre-paration showed an electrophoretic diagram including two or three peaks, which is similar to that of the actomyosin-like protein from salt extract of the squid muscle. Also in respect of the electrophoretic mobility each component corresponded with each other in both cases. So the two actomyosin-like protein, the one from aqueous extract and another from salt extract of the squid muscle, including the accompanying proteins, were found to be identical. There was found an indication that Component 2, the middle-speeded is rather scanty in the case with the protein from aqueous extract.
2) When a precipitate of the actomyosin-like protein from salt-extract was extracted repeatedly with water, a better part of the protein moved into solution. Moreover, marked streaming birefringence (SB) was found in the extracts as the case with the repeated aqueous extraction of the squid muscle. On the contrary, SB was never found in the case with carp actomyosin, whereas nearly half of the used actomyosin of the carp was brought in solution.
3) From these experimental bases, it was proposed that the actomyosin-like protein which occupies the dominant part of the squid muscle proteins should be referred to as “M-actomyosin” no matter whether it is isolated from salt extract or from aqueous extract.