OYA et al1, 2) determined the optimum conditions of autolysis of various fish muscles in the presence of a toluene-chloroform mixture or thymol, but did not examine the effect of these antiseptics on the autolysis of fish muscle.
In the present work, the autolysis of mackerel muscle was investegated under the strictly aseptic conditions without the addition of any antiseptic. The results obtained were as follows.
1. The autolysis of the mackerel muscle homogenate proceeded readily at 45°C at pH 3.5 and at 60-65°C in a slightly alkaline pH range (Figs. 1-7).
2. Antiseptics such as toluene, chloroform, and thymol did not affect the optimum pH (3.5) of autolysis at 45°C but considerably lowered the rate of autolysis (Figs. 8 and 9).
3. These antiseptics inhibited the brown discoloration and fat oxidation of the mackerel muscle homogenate during the autolysis (Table 1).