魚の生体外排卵に関する生物学的研究-III.
サケの脳下垂体性ゴナドトロビンのメダカの生体外排卵に及ぼす効果について
1972 年 38 巻 2 号 p. 97-100
詳細
1972 年 38 巻 2 号 p. 97-100
This study was carried out to examine the effects of partially purified salmon (Oncorhynchus tshawytscha) gonadotropin (SG-G100) on ovulation in vitro in Oryzjas latipes. Incubation of oocytes with 0.04-400μg/ml SG-G100 was initiated at 10.00 a.m, 5.00 p.m. and 11.00 p.m. and the percentage of oocytes which had ovulated was determined at 10.00 a.m. on the following day. In the 10.00 a.m. group slight ovulation was observed at the 4μg/ml dose. In the 5.00 p.m. group, ovulation occurred at all dosages from 0.04μg/ml to 400μg/ml gonadotropin, with maximum ovulation (67%) at 0.4μg/ml SG-G100. Luteinizing hormone (NIH-LH-S16) was less effective than SG-G100. In the 11.00 p.m. group there was 19% ovulation in the control group and the most effective dosage of SG-G100 was 4μg/ml, which induced 60% ovulation.
