魚類血清蛋白の電気泳動的,免疫学的分析

抄録

The sera of five species of Salmonidae, three of Cyprinidae and one of Anguil-lidae were analyzed by means of cellulose acetate membrane electrophoresis and immunoelectrophoresis. The sera of some species were studied by salt fractionation and gel filtration with Sephadex G-200.
Electrophoresis revealed obvious species differences and individual variation. Most of the species showed only a minor γ-globulin fraction. The electrophoretic patterns of albumin were quite different between the Salmonidae and Cyprinidae; Salmonidae showed one or two clear bands while Cyprinidae showed an indistinct diffuse band.
Individual variations in chum salmon and rainbow trout were observed primarily in the albumin fraction, but in eel variations were observed mostly in the globulin fraction. Some proteins, which are considered to be related to egg components, appeared in the β region of chum salmon, kokanee salmon and rainbow trout.
Distinct species differences were also revealed by immunoelectrophoresis. The sera of species of the family Salmonidae revealed about nine precipitin lines with anti-chum salmon and anti-rainbow trout sera, but very few with anti-carp serum. On the other hand, fish of the family Cyprinidae revealed about seven precipitin lines with anti-carp, but showed only one with anti-chum salmon and anti-rainbow trout sera.