コイ血合肉と肝膵臓ミトコンドリアにおける18:1ω9酸の酸化による3:0酸生成の検討
1981 年 47 巻 1 号 p. 79-83
詳細
1981 年 47 巻 1 号 p. 79-83
In order to elucidate the pathway of unsaturated fatty acid oxidation in carp, Cyprinus carpio, dark muscle and hepatopancreas mitochondria, the products formed by oxidation were studied using U-14C-18:1ω9 acid as substrate.
Although O2 uptake due to oxidation of 3:0 acid was clearly observed in carp dark muscle mitochondria, as well as those of 2:0, 4:0 and 18:1ω9 acids, 3:0 acid and methylmalonic acid were not detected as the oxidation products of 18:1ω9 acid in carp dark muscle and hepatopancreas mitochondria; whereas acetic, fumaric and succinic acids were formed. Moreover, addition of HCO-3 to the medium did not increase the difference in O2 uptake due to oxidation between 18:1ω9 and 16:0 acids both in carp dark muscle and hepatopancreas mitochondria.
This suggested that 3:0 acid was not formed during the oxidation of unsaturated fatty acid in carp dark muscle and hepatopancreas mitochondria. Consequently, the author concluded that the oxidation of unsaturated fatty acid as energy source in carp essentially fell under the category of β-oxidation.
