Two types of the fresh water shrimp Palaemon paucidens DE HAAN inhabiting the Natori River system (Japan) were genetically separated by starch gel electrophoresis. One type, A, consists of the individuals exhibiting either GpiA, MpiA or Mdh-2A but not Mdh-1c genes, while the other type, B, does not exhibit GpiA, MpiA or Mdh-2A but shows Mdh-1c in moderate frequency. No evidence of hybridization between A and B types was observed even in areas where both were found together throughout the breeding season. Significant differences between the A and B types were found, i.e., the individuals of A type lay large eggs and most of them possess 2 lower rostrum teeth, while the individuals of B type lay small eggs and most of them possess 3 lower teeth.