抄録
Alternative complement pathway activity (ACH50) of porgy serum was measured by in-cubating the serum with rabbit red blood cells (RaRBC) in the presence of 10mM EGTA. It was confirmed that the concentration of EGTA (10mM) is enough to prevent the activation of the classical pathway of porgy complement, and that among red blood cells from several animals, RaRBC is the most effective activator of the alternative pathway of porgy comple-ment.
Optimum conditions for the hemolytic reaction were: reaction temperature, 15°C; reaction time, 120min; pH 7.8; concentration of Mg2+, 10mM. When determined under these conditions, the ACH50 values of the sera from 33 porgy, weighing 23-31g, were remarkably high (143±81units/ml) as compared with those reported for mammalian sera.
