コイ血漿リポタンパク質の分離とその性状

抄録

VLDL (d<1.006g/ml), LDL₁(d 1.006-1.063g/ml), LDL₂ (d 1.063-1.125g/ml), and HDL (d 1.125-1.21g/ml) were isolated by sequential ultracentrifugation from carp plasma obtained at 8h after feeding and purified by gel-filtration column chromatographies. Carp VLDL, LDL₂, and HDL were all detected as a single band, but LDL₁ consisted of three bands with lipid staining by polyacrylamide gel electrophoresis. The major lipid components of VLDL and LDL₁ were triglycerides. Moreover, both VLDL and LDL₁ contained human apo B-, apo A-II-, and apo C- like proteins. Carp LDL₂ contained relatively high levels of sterol esters in addition to trig-lycerides, and contained exclusively human apo B-like protein. Carp HDL, which was the pre-dominant class in all lipoproteins, contained phospholipids as the major lipid components and human apo A-I-and A-II-like proteins as the major apoprotein components.