Detection of Immunoglobulins (γM and γG) by Fluorescent Antibody Method in Canine Lnng Allotransplantation

Abstract

The histochemical examination by the fluorescent antibody method for demonstrating the localization of immunoglobulins (γM and γG) in lung allografts and other lymphoid tissues of the recipients was performed in canine lung allotransplantation. Histologic and immunohistologic observations of the transplanted lungs of the non-treated group seemed to be characterized by initial appearance of pyroninepositive cells and γM-containing cells around the small vessels and bronchioli. In the graft of the treated group, appearance of pyronine-positive cells and of γM positive cells was most dominant at the time of rejection. According to immunofluorescent stain, γM- and γG-positive cells were apparently observed in the lymphoid tissues of the recipient at the time of rejection. Accordingly. γM-containing cells seemed to play an important role in the rejection of the lung transplant.