抄録
The bacterial cell fractions obtained from the supernatant liquor of digesters for human excreta and distillers' wastes are noteworthy vitamin B_<12> resources. Bioautographic identification and characterization of vitamin B_<12> and its related factors were investigated in the bacterial cell fractions above mentioned for the purpose of utilization for industrial raw materials of the vitamin. Water-saturated sec-butanol containing a trace of cyanide with or without acetic acid or potassium perchlorate was used as a developing agent. Since some of vitamin B_<12> factors have similar Rf values it seems to be difficult to separate them completely each other and identify them bioautographically. However, it was found that the bacterial cell fractions examined contained other vitamin B_<12> factors than cyanocobalamin, and that for complete separation and identification of vitamin B_<12> factors, other appropriate techniques were necessary than paper chromatography with bioautography.
