抄録
In the previous paper, the author reported that ethylthiamine showed almost the same grade of growth promoting effect on rats as thiamine but the liver extracts of these animals showed only low cocarboxylase activity in spite of the fact the extracts contained relatively large amounts of so-called bound thiamine as determined by the thiochrome method. This was interpreated that ethylthiamine pyrophosphate was synthesized in the animal tissue and the pyrophosphate had a weak activity as cocarboxylase. In the present report, consequently, pure samples of ethylthiamine pyrophosphate were synthesized chemically and their cocarboxylase activity was measured manometrically. It was revealed that the synthetic samples had 1/12 to 1/15 of the activity of cocarboxylase.
