抄録
For the purpose of ascertaining the role of vitamin A in vision, vitamin A alcohol and ester in retina and pigment layer were estimated by means of alumina chromatography and glyceroldichlorohydrin method. Furthermore the difference of their amounts in the dark and light adaptation was observed. It was assumed that use of 1N KOH・90% ethanol in an atmosphere of nitrogen was desirable for the extraction of vitamin A from retina or pigment layer. Most of vitamin A in retina and pigment layer was found to be alcohol form. In dark adaptation, a decrease of vitamin A alcohol and a slight increase of its ester were observed. On the alumina chromatogram, a yellow layer, differenciated from vitamin A, was found, which was assumed to be a carotenoid compound.
