抄録
Synthesis of heteropyrithiamine by condensation of hydroxymethylpyrimidine diphosphate (OMP-PP) and pyridine was applied to the chemical determination of OMP-PP. The heteropyrithiamine formed after heating at 90℃ for 30 minutes with OMP-PP and 1 M pyridine in a final concentration was estimated by alkaline ferricyanide oxidation according to Nose. The reaction product showed a maximal fluorescence at pH 7.0-8.0 with tris buffer. Employing this method, the quantities of OMP-PP ranging from 0.01 to 0.1 μmole can be determined. OMP-monophosphate reacted also with pyridine with a sensitivity about 1/20 fo OMP-PP. The enzymatic OMP-PP formation by thiamine synthesizing enzyme was found to be activated by 64 % after adding NaF to the reaction mixture.
