抄録
A new medium containing no sugar was invented for the mixed culture of Clostridium thiaminolyticum and Lactobacillus bifidus. Clostridium and Lactobacillus grew well without morphorogical change in this medium, as in their suitable medium, on the separated cultures. On mixed culture of the both bacteria in the new medium, Clostridium grew well as in the separated culture, while the growth of Lactbacillus was suppressed a little. On the separated culture, final pHs of the culture of Clostridium and Lactobacillus were 7.2 and 4.8,respectively. In the case of mixed culture of the two, almost median value of pH 7.2 and 4.8 was maintained during the cultivation. Thiaminase activity of Clostridium in the mixed culture decreased about to one half of that in separated culture, while the cell number did not differ in mixed or separated culture within 4 to 5 days. Thus it seems that the growth of Clostridium thiaminolyticum, as well as Bacillus thiaminolyticus or B. aneurinolyticus, is not suppressed by the presence of Lactobacillus bifidus.
