抄録
A fluorometric procedure for the fractional determination of O-acylthiamine derivatives and thiamine was established by the use of sodium or potassium carbonate as alkali reagent in cyanogen bromide oxidation. Some factors affecting the accuracy of determination were tested and it was found that the salt concentration of the oxidation mixture was an important one. A standard procedure for the mixture of thiamine, OBT, TDS, BTDS were described in table 7. Usefulness and versatility of the acylthiochrome fluorometry were briefly discussed.
