抄録
Kinetic and experimental studies were made on the exchange reaction of acyl-TDS, TAD and TGD with GSH, PA-SH and Hb-SH, respectively. Second order reaction rate constants were estimated at pH 7.30,37℃ in vitro, as listed in table 2,suggesting the role of GSH for the main reactant in erythrocytes. Addition of TAD or acyl-TDS to intact human or rabbit erythrocytes suspension caused the rapid consumption of GSH and the slower decrease of Hb-SH in erythrocytes. The GSH consumed was found to be reproduced within five to ten minutes, and the mechanism was deduced to be an action of glutathione reductase. On intravenous injection of 25 mg/kg BuTDS in rabbit the similar changes in blood GSH content were also observed. From these results, metabolism of the disulfide type thiamine derivatives in blood was discussed.
