Abstract
In the high-performance liquid chromatographic (HPLC) determination of vitamins A, E and ubiquinone (UQ) in the biological materials, the microparticle columns were used in place of the porous layer beads columns which had been used in our previous reports, and then higher theoretical plate numbers, better resolution factors and less coefficient of variation on the microparticle columns were obtained. This HPLC method could be easily applied to the determination of vitamins A and E, but not to UQ in serum, in which β-carotene like substances interfered the UQ peaks. Furthermore, the simple HPLC method was established for the simultaneous determination of vitamins A and E using NH_2 microparticle column in combination with on-column concentration.
