実験的糸球体腎炎ラットにおける尿中Prostaglandin Eの排泄について

抄録

The change of urinary prostaglandin E (PGE) excretion was studied in experimental glomerulonephritic rats (Masugi nephritis), and the effects of ouabain and chlorothiazide on urinary PGE excretion in nephritic rats were examined. Experimental glomerulonephritis was induced in rats by the intravenous injection of anti-kidney serum obtained from rabbits immunized with the homogenate of rat kidney. The urinary PGE was measured by the bioassay using rat stomach fundus strip. Urinary PGE and urinary electrolytes in nephritic and normal rats were determined. There was positive relationship between urinary PGE and urinary electrolytes in both kinds of rats. The urinary PGE and sodium excretion was increased in nephritic rats by ouabain (10 mg/kg), but not in normal rats. The potassium and chloride excretion was significantly increased in both nephritic and normal rats by ouabain. The diuretic effect of chlorothiazide (15 mg/kg) was more significantly recognized in nephritic rats than in normal rats. But there was little change in urinary PGE excretion by chlorothiazide in both nephritic and normal rats. The reduction of urinary PGE excretion in nephritic rats may be due to a decrease of PGE biosynthesis in the renal medulla and/or a diminished secretion of PGE into renal tubules, both accompanied with the disturbance of glomerular function.