抄録
Paper chromatographic examination of thiamine crystal revealed the presence of three spots, (A), (B), and (C), besides that of thiamine. The absorption spectrum, diazo reaction, and thiochrome reaction of (B) were closely similar to those of thiamine and its hydrolysis with hydrochloric acid gave a compound showing activity against Lactobacillus fermenti-36. It is therefore assumed that (B) is acetylthiamine. The absorption spectrum of (C) in aqueous solution of pH 3.0 was similar to that of thiamine but that in 2% sodium hydroxide solution was similar to those of chloroethylthiamine and thiamine anhydride in the same solvent. Consequently, (C) is probably chloroethylthiamine or bromoethylthiamine.
