スルフイソキサゾールの体内変化に関する研究 (第3報)

スルフイソキサゾールの尿中濃度測定法およびその排泄について

抄録

A method was devised to isolate and determine sulfisoxazole alone from various metabolic products formed after administration of sulfisoxazole. The procedure is as follows:
A chromatographic column is prepared with 1g. of powdered cellulose, filled in a glass tube (5.5mm.×20cm.) with a solvent mixture of butanol-propanol-water (2:1:1). The sample solution is diluted five fold with butanol-propanol mixture (1:1) and 0.5cc. of its supernatant is passed through the foregoing column. The column is eluted with a solvent mixture of butanol-propanol-water (2:1:1) and 3.5cc. of the initial eluate is discarded. The next 2cc. of the effluent is diluted with ethanol to 5cc. and 1cc. of this solution is added with 0.3cc. of 2N hydrochloric acid and 5cc. of 2% ethanolic solution of p-(dimethylamino)benzaldehyde. The mixture is allowed to stand at 30° for 7minutes, diluted to 10cc. with ethanol, and its optical density at 453mμ is measured. Concentration of sulfisoxazole is calculated from the calibration curve prepared preliminarily.
The amount of unchanged sulfisoxazole excreted into urine after oral administration of this sulfa drug was determined. Comparative examination was also made on the present method and that of Bratton-Marshall, and the amount of sulfisoxazole changed in the body was measured.