Bile-sensitive Lipaseの研究(第7報) : 界面活性剤のMucor Lipaseにおよぼす影響 酵素剤の研究 第48報

抄録

The biological effects of surfactants are only partially understood. To clear some of mechanisms of enzyme activation and inhibition by surfactants, the effect of surfactants of various types on Mucor lipase was examined. Anionic surfactants had a strong inhibitory effect on the lipase in both of enzyme system and reaction system. However, surfactants in a certain concentration partially accelerated lipolysis and this concentration was generally equivalent to one-half the critical micelle concentration (c.m.c.) in a reaction mixture. All cationic surfactants were found under certain conditions to enhance the rate of hydrolysis of olive oil. The concentration of surfactants which gave the most powerful stimulation was 0.0055% (w/v) for benzalkonium chloride, 0.0065% for benzethonium chloride and 0.004% for cetylpyridinium chloride in the reaction system, but more concentrated solutions of them caused inhibition. The increase of hydrolysis rate was found to depend essentially on the oil/surfactant ratio, and a small amount of the surfactant caused an increase in the lipase adsorption at oil-water interface. Both nonionic and amphoteric surfactants enhanced the rate of lipolysis till their concentration reached about one-half the c.m.c. However, the surfactants in c.m.c. and more concentrated solution caused strong inhibition. The preincubation of the enzyme with either nonionic or amphoteric surfactant solution resulted in the increase of the lipase activity and its adsorption at oil-water interface.