Bacillus属細菌の産生する生理活性物質の研究(第3報) : B.subtilis Kのにより産生される家兎血清カルシウム低下性物質の単離とその性質

抄録

A serum calcium-decreasing principle was isolated in homogenous state from the culture filtrate of Bacillus subtilis K without damage of its biological activity, using acrylamide gel electrophoresis by means of electrofocusing on Carrier Ampholyte, and it was named α-factor. As a result of chemical analysis, the principle was considered to be a lipoprotein, and 17 kinds of amino acid were detected as the componental amino acids in protein moiety of α-factor. The N-terminal and C-terminal amino acids in α-factor were identified as leucine and serine, respectively. This substance not only lowered the serum calcium level but also increased markedly the inorganic phosphorus level in serum and the citric acid level in blood of a rabbit, and prevented the urinary excretion of calcium in a rabbit. Moreover, the calcium decreasing activity of the radioiodinated α-factor preparation was largely retained the characteristically a large amount of radioactivity was demonstrated in the thyroid, hypophysis, parotid, and submaxillary glands which are related to calcium metabolism in mammals, even at 3 hr after the administration of ¹³¹I-α-factor to a rabbit.